Name
Recombinant Human EphB2 / Hek5 Protein 100µg
Catalog number
PKSH031440-100µg
Size
100µg
Price
626 EUR
Fusion tag
NA
AP Mol Mass
66 kDa
Mol Mass
59 kDa
Accession
NP_059145.2
Group
recombinants
Expressed Host
HEK293 Cells
Sequence
Met 1-Leu 543
Source
Recombinants or rec. proteins
Purity
＞95 % as determined by SDS-PAGE
Synonym
CAPB;DRT;EK5;EPHT3;ERK;Hek5;PCBC;Tyro5
Reconstitution
Please refer to it for detailed information.
Endotoxin
＜1.0 EU per µg as determined by the LAL method
Formulation
Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0
Activity
Immobilized human EPHB2 at 10 μg/ml (100 μl/well) can bind human EFNB2-Fch, The EC50 of human EFNB2-Fch is 18.2-42.7 ng/ml.
Stability and Storage
Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Background
Ephrin type-B receptor 2, also known as EphB2, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA10, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. EphB2 receptor tyrosine kinase phosphorylates syndecan-2 and that this phosphorylation event is crucial for syndecan-2 clustering and spine formation. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs). Ligand-mediated activation of Ephs induce various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer. EphB receptor tyrosine kinases are enriched at synapses, suggesting that these receptors play a role in synapse formation or function. We find that EphrinB binding to EphB induces a direct interaction of EphB with NMDA-type glutamate receptors. This interaction occurs at the cell surface and is mediated by the extracellular regions of the two receptors, but does not require the kinase activity of EphB.