Recombinant Human EphB2 / Hek5 Protein 100µg

Recombinant Human EphB2 / Hek5 Protein 100µg

Name

Recombinant Human EphB2 / Hek5 Protein 100µg

Catalog number

PKSH031440-100µg

Size

100µg

Price

626 EUR

Fusion tag

NA

AP Mol Mass

66 kDa

Mol Mass

59 kDa

Accession

NP_059145.2

Group

recombinants

Expressed Host

HEK293 Cells

Sequence

Met 1-Leu 543

Source

Recombinants or rec. proteins

Purity

>95 % as determined by SDS-PAGE

Synonym

CAPB;DRT;EK5;EPHT3;ERK;Hek5;PCBC;Tyro5

Reconstitution

Please refer to it for detailed information.

Endotoxin

<1.0 EU per µg as determined by the LAL method

Formulation

Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0

Activity

Immobilized human EPHB2 at 10 μg/ml (100 μl/well) can bind human EFNB2-Fch, The EC50 of human EFNB2-Fch is 18.2-42.7 ng/ml.

Stability and Storage

Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Background

Ephrin type-B receptor 2, also known as EphB2, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA10, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. EphB2 receptor tyrosine kinase phosphorylates syndecan-2 and that this phosphorylation event is crucial for syndecan-2 clustering and spine formation. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs). Ligand-mediated activation of Ephs induce various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer. EphB receptor tyrosine kinases are enriched at synapses, suggesting that these receptors play a role in synapse formation or function. We find that EphrinB binding to EphB induces a direct interaction of EphB with NMDA-type glutamate receptors. This interaction occurs at the cell surface and is mediated by the extracellular regions of the two receptors, but does not require the kinase activity of EphB.