Name
EPHB2 (48CT12.6.4) Monoclonal Antibody
Catalog number
bsm-51269M
Size
0.1ml
Price
211 EUR
Modification(s)
None
Gene ID number
2048
Target Antigen
EPHB2
Cross reactivity
Human
Swiss Prot
P29323
French translation
anticorps
Clone number
48CT12.6.4
Clonality
Monoclonal
Conjugation
Unconjugated
Tested Applications
WB, FCM, IHC-P
Category
Primary Antibodies
Modification site(s)
Unmodified antibody
Recommended dilutions
WB(1:1000), FC(1:10~50), IHC-P(1:200)
Long name
EPHB2 (48CT12.6.4) Monoclonal Antibody
Also known as
EPHB2 (48CT12.6.4) Monoclonal Antibody
Specificity
This is a highly specific antibody against EPHB2.
Cross reactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
Source
Purified His-tagged EPHB2 protein(Fragment, between amino acids 127~425) was used to produce this monoclonal antibody.
Storage conditions
Keep the antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for up to 1 year.
Properties
If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Purification method
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
Synonym names
DRT; EK5; ERK; CAPB; Hek5; PCBC; EPHT3; Tyro5; Ephrin type-B receptor 2; Developmentally-regulated Eph-related tyrosine kinase; ELK-related tyrosine kinase; EPH tyrosine kinase 3; EPH-like kinase 5; Renal carcinoma antigen NY-REN-47; Tyrosine-protein kinase TYRO5; Tyrosine-protein kinase receptor EPH-3; EPHB2; EPTH3
Background of the target antigen
Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Functions in axon guidance during development. Involved in the guidance of commissural axons, that form a major interhemispheric connection between the 2 temporal lobes of the cerebral cortex. Also involved in guidance of contralateral inner ear efferent growth cones at the midline and of retinal ganglion cell axons to the optic disk. In addition to axon guidance, also regulates dendritic spines development and maturation and stimulates the formation of excitatory synapses. Upon activation by EFNB1, abolishes the ARHGEF15-mediated negative regulation on excitatory synapse formation. Controls other aspects of development including angiogenesis, palate development and in inner ear development through regulation of endolymph production. Forward and reverse signaling through the EFNB2/EPHB2 complex regulate movement and adhesion of cells that tubularize the urethra and septate the cloaca. May function as a tumor suppressor.