Name
Rabbit anti ERK/MAPK (Thr202/Tyr204)
Catalog number
X1670P
Size
10MinibLots
Price
398 EUR
Antibody is raised in
Rabbit
Storage
-80ºC
Protein number
see ncbi
Clone
Polyclonal
Antibody's specificity
No Data Available
Category
Primary Antibodies
Latin name
Oryctolagus cuniculus
Application
Western Blot, Dot Blot
Antibody's reacts with
Human, Mouse, Rat, Xenopus
Antigen-antibody binding interaction
Rabbit anti ERK/MAPK (Thr202/Tyr204) Antibody
Antibody's reacts with these species
This antibody doesn't cross react with other species
Antibody come from
Fusion protein from the N-terminus of rabbit renal Na+/Ca2+ exchanger
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Other description
Lyophilized (See usage for reconstitution). Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.
Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
Long description
Extracellular-Signal Regulated Kinase/Mitogen-Activated Protein Kinase (ERK/MAPK) is an integral component of cellular signaling during mitogenesis and differentiation of mitotic cells and also is thought to play a key role in learning and memory. The activity of this kinase is regulated by dual phosphorylation at Thr202 and Tyr204.
Antibody's suited for
Antibody can be used for Westen blot (1:1000 dilution). Optimal concentration should be evaluated by serial dilutions. This lyophilized antibody can be stored at -20¼C and is stable for at least 2 years. The antibody should be reconstituted in 50µl PBS before use. After reconstitution the antibody should be aliquoted and stored at -20C.
About
Rabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Relevant references
1. Bano D, Young KW, Guerin CJ, Lefeuvre R, Rothwell NJ, Naldini L, Rizzuto R, Carafoli E, Nicotera P (2005) Cleavage of the plasma membrane Na+/Ca2+ exchanger in excitotoxicity. Cell 120:275-285._x000B__x000B_2. Choi DW (2005) Neurodegeneration: cellular defences destroyed. Nature 433:696-698._x000B__x000B_3. Hagihara H, Yoshikawa Y, Ohga Y, Takenaka C, Murata KY, Taniguchi S, Takaki M (2005) Na+/Ca2+ exchange inhibition protects the rat heart from ischemia-reperfusion injury by blocking energy-wasting processes. Am J Physiol Heart Circ Physiol 288:H1699-H1707._x000B__x000B_4. Matsuoka S, Nicoll DA, Reilly RF, Hilgemann DW, Philipson KD (1993) Initial localization of regulatory regions of the cardiac sarcolemmal Na+/Ca2+ exchanger. Proc Natl Acad Sci USA 90:3870-3874.